PepID Biopeptide Libraries
Biopeptide, Biosynthetic peptides, Multipeptid-Expression,
PepIdent separates hosting and expression of peptide libraries into 2 subsystems. It is a biopeptide system because the peptides are produced in a host organisms (bacteria or the like) from a plasmid DNA construct.
In a nutshell, this is how PepIdent works:
First, your protein sequence of interest is partitioned into linear peptides of defined length and overlap - according to your needs. Our Peptide Design Tools automatically generates the initial library design but our staff will be happy to assist you if you run into problems, e.g. with defining the right restriction sites that are compatible with our target expression vector or figuring out the best overlap or which design best matches your research agenda.
The peptide-coding DNA sequences are then assembled into a single or a set of compact constructs. These are then synthesized de novo and hosted on the source / back-up vector from which they can be released whenever necessary.
Peptides are released by simply cutting with a unique combination of restriction enzymes and then cloned into the expression vector. The expression vector can carry your required tag, e.g. GST. This will result in a fusion peptide ready for expression testing and utilization in your application.
, Biosynthetic peptides