ToggleTec Multi-Gene/Protein Expression Systems
Heterologe Expression, multigene expression, Multigenexpression,
TogTec flexiColi and flexiBac
+ two acceptor and two donor vectors for manipulation of DNA in E. coli plus - in the case of MultiBacTurbo - a bacmid for transfer of your final construct(s) into baculoviruses and subsequent trans-fection of your insect cells of choice.
+ build multigene concatemers via toggle cloning
+ vectors with features that allow you to flexibly insert and recombine your gene(s) of interest into (multi-) gene expression cassettes before assembling your final construct in E. coli.
+ comes with bacterial strains for propagation of the donor vectors and acceptor-donor fusions; separate strains carrying bacmids for transfection of insect cells are supplied
+ express single or multiple proteins simultaneously
+ assemble multi-gene cassettes easily and rapidly
+ generate and test gene variants or mutants rapidly and in parallel to speed up and quickly fine-tune/adapt your gene expression project
+ fuse single or multigene acceptor and donor plasmids via Cre recombination
+ easy blue/white screening for recombinant bacmids, no plaque purification necessary, zero background
+ improve complex stability and solubility through co-expression of partnering proteins
+ automate your entire multigene expression vector production process for high through-put and scale-up
, multigene expression
, Toggle cloning